p90Rsk/RSK1/RPS6KA1 Rabbit pAb - Q15418

Core Product Specifications and Parameters

Biological Background: p90Rsk/RSK1/RPS6KA1 Function and Localization

• Ribosomal protein S6 kinase alpha-1 (RPS6KA1), also known as p90Rsk, RSK1, or MAPKAPK1, is a serine/threonine-protein kinase that acts downstream of ERK1/2 signaling to mediate mitogenic and stress-induced cellular responses. Related references: PMID:10679322 PMID:12213813 PMID:15117958
• The kinase regulates transcription by phosphorylating and activating transcription factors such as CREB1, ETV1/ER81, and NR4A1/NUR77, thereby promoting expression of immediate-early genes in response to growth factors. Related references: PMID:18508509 PMID:18813292 PMID:12213813 PMID:16223362
• p90Rsk promotes protein translation by phosphorylating ribosomal protein S6 (RPS6) via an mTOR-independent mechanism and by enhancing EIF4B affinity for the EIF3 complex, thus stimulating cap-dependent translation. Related references: PMID:17360704 PMID:16763566
• It plays a key role in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 and RPTOR, regulating mTORC1 activity, and feedback-controlling mTORC2 via DEPTOR phosphorylation. Related references: PMID:15342917 PMID:22017876
• p90Rsk mediates cell survival by phosphorylating pro-apoptotic proteins BAD and DAPK1, suppressing their apoptotic functions, and also promotes hepatic stellate cell survival through CEBPB phosphorylation. Related references: PMID:10679322 PMID:16213824 PMID:11684016
• In cell cycle regulation, it phosphorylates the CDK inhibitor CDKN1B, promoting its association with 14-3-3 proteins and preventing nuclear translocation, thereby facilitating G1 progression. Related references: PMID:18508509 PMID:18813292
• p90Rsk regulates cell migration by phosphorylating EPHA2 at Ser-897, a signaling event critical for EPHA2-mediated cell motility. Related references: PMID:26158630
• The protein localizes to both the nucleus and cytoplasm and contains two kinase domains, belonging to the AGC family of kinases. It is subject to phosphorylation and alternative splicing, and interacts with viral components during HHV-8 infection.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to the C-terminus of human p90Rsk; therefore, the antibody may recognize full-length and C-terminal fragments of RPS6KA1.
• For Western blotting, consider using whole-cell lysates from human, mouse, or rat cells; the predicted molecular weight is approximately 83 kDa, though post-translational modifications may cause band shifts.
• In immunohistochemistry, optimize antigen retrieval and antibody dilution, and validate staining in known positive tissues.
• For immunocytochemistry, fixation and permeabilization should be carefully controlled to preserve both nuclear and cytoplasmic localization.
• This polyclonal antibody is suitable for ELISA-based detection; pairing with a suitable capture antibody may enable quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This p90Rsk/RSK1/RPS6KA1 antibody supports studies in MAPK signaling, cell proliferation, and apoptosis—key areas for oncology and metabolic disease diagnostics. To accelerate your assay development, CamelBio offers one-stop technical and procurement support from concept to clinic.