p75NTR/NGFR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08138

Core Product Specifications and Parameters

Biological Background: p75NTR/NGFR Function and Localization

• p75NTR/NGFR (also known as CD271, TNFRSF16, or Gp80-LNGFR) is a member of the tumor necrosis factor receptor superfamily, functioning as the low-affinity nerve growth factor receptor.
• It binds to neurotrophins NGF, BDNF, NTF3, and NTF4, and forms a heterodimeric receptor with SORCS2 that preferentially binds precursor forms of NGF, BDNF, and NTF3 with high affinity. Related references: PMID:24908487
• Plays an important role in differentiation and survival of specific neuronal populations during development, and can mediate both cell survival and cell death of neural cells.
• Involved in the inactivation of RHOA, affecting cytoskeletal dynamics. Related references: PMID:26646181
• Regulates the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by modulating RAB31 activity, thereby contributing to insulin-dependent glucose uptake.
• Necessary for the circadian oscillation of clock genes BMAL1, PER1, PER2, and NR1D1 in the suprachiasmatic nucleus and liver, as well as genes involved in glucose and lipid metabolism in the liver. Related references: PMID:23785138
• Together with BFAR, negatively regulates NF-kappa-B and JNK-related signaling pathways. Related references: PMID:22566094
• Subcellular localization includes the cell membrane, cytoplasm, perikaryon, growth cones, and dendritic spines, consistent with its diverse roles in signaling and neuronal development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 273–427), potentially allowing detection of full-length and certain isoforms; verify in relevant sample systems.
• For Western blot, the predicted molecular weight is ~45 kDa; however, glycosylation may cause band shifts—include appropriate controls.
• Polyclonal antibodies can enhance signal in ELISA; consider using this antibody as a detection reagent in sandwich ELISA after pairing validation.
• For immunofluorescence, optimize fixation/permeabilization protocols to preserve membrane and cytoplasmic localization signals.
• Cross-reactivity with mouse and rat suggests conserved epitopes; validate in the specific model organism before drawing comparative conclusions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories, providing ready access to high-quality antibodies like this p75NTR/NGFR polyclonal antibody. For programs targeting neurotrophin signaling, apoptosis, or metabolic rhythms, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—helping you accelerate from concept to clinic.