P2RX4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99571

Core Product Specifications and Parameters

Biological Background: P2RX4 Function and Localization

• P2RX4 encodes the P2X purinoceptor 4, an ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium, and calcium (PubMed:9016352). Related references: PMID:9016352
• Plays pivotal roles in immunity and central nervous system physiology, including T-cell activation, Th17 cell differentiation via RORC expression (PubMed:35165166), and microglia motility. Related references: PMID:35165166
• Localizes to the cell membrane and lysosome membranes, functioning as both a cell-surface and intracellular ligand-gated ion channel.
• Contains key structural features typical of P2X receptors: two transmembrane helices, disulfide bonds, and glycosylation sites; binds ATP as a ligand.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.
• Weakly activated by CTP, but not GTP or UTP (By similarity).

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is 43kDa; however, post-translational modifications such as glycosylation may cause band shifts. Validate in your sample system.
• For immunofluorescence (IF/ICC), consider fixation and permeabilization conditions to detect both surface and intracellular pools; live-cell labeling may be used for surface localization studies.
• This polyclonal antibody may be used in ELISA applications; consider pairing with a detection antibody for sandwich ELISA formats, and validate specificity with appropriate controls.
• The immunogen spans a large extracellular domain (aa 55-338), which may generate antibodies recognizing native epitopes; however, each new lot should be tested in the intended assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this anti-P2RX4 antibody, supporting projects in ion channel biology, immunology, and neuroscience. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development and scale-up. Partner with CamelBio to bridge the gap from concept to clinic with reliable raw-material supply solutions.