P23/PTGES3 Rabbit mAb for WB, IF/ICC, ELISA - Q15185

Core Product Specifications and Parameters

Biological Background: P23/PTGES3 Function and Localization

• PTGES3 (prostaglandin E synthase 3), also known as cPGES, p23, and TEBP, is a multifunctional protein encoded by the PTGES3 gene in humans. It belongs to the GST superfamily and functions both as a prostaglandin synthase and a molecular chaperone.
• As a cytosolic prostaglandin synthase, PTGES3 catalyzes the conversion of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2), a key lipid mediator involved in inflammation and pain signaling. Related references: PMID:10922363
• Acting as a co-chaperone, PTGES3 interacts with HSP90 and localizes to genomic response elements in a hormone-dependent manner, disrupting receptor-mediated transcriptional activation by promoting disassembly of transcriptional regulatory complexes. Related references: PMID:11274138, PMID:12077419
• PTGES3 facilitates the hydroxylation of HIF alpha proteins by recruiting EGLN1/PHD2 to the HSP90 pathway, thereby influencing the cellular response to hypoxia. Related references: PMID:24711448
• Subcellularly, PTGES3 is predominantly cytoplasmic, consistent with its roles in prostaglandin synthesis and chaperone machinery.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond and Ubl conjugation), which may regulate its activity and stability. The protein also undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human PTGES3. For Western blot (WB), denaturing and reducing conditions are typically effective due to the linear epitope nature of the peptide immunogen.
• In immunofluorescence (IF/ICC), expect cytoplasmic staining; fix and permeabilize according to the target cell line's requirements.
• For ELISA, consider direct or sandwich formats using recombinant protein or peptide as the capture antigen; cross-reactivity with mouse and rat PTGES3 suggests utility in rodent models.
• Given its chaperone role, PTGES3 may interact with partner proteins. Validate antibody performance under native conditions if used for co-immunoprecipitation (Co-IP).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw material procurement, providing validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline diagnostic development. This anti-PTGES3 antibody supports inflammation and cancer research; complement it with CamelBio's full workflow sourcing—from rare target raw materials to optimized assay components—accelerating your concept-to-clinic progress.