OXCT1 (SCOT) Rabbit Polyclonal Antibody for WB, ELISA - P55809

Core Product Specifications and Parameters

Biological Background: OXCT1 Function and Localization

• OXCT1 encodes the mitochondrial enzyme succinyl-CoA:3-ketoacid coenzyme A transferase 1, also known as SCOT or 3-oxoacid CoA-transferase 1. Multiple isoforms are generated via alternative splicing.
• This enzyme catalyzes the first, rate-limiting step in ketone body utilization, transferring coenzyme A from succinyl-CoA to acetoacetate to form acetoacetyl-CoA, which enters the citric acid cycle for energy production. Related references: PMID:10964512
• The enzyme functions as a dimer; only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate, and the reaction proceeds via an unstable anhydride intermediate.
• OXCT1 is strictly localized to the mitochondrial matrix, consistent with its role in ketone body catabolism.
• Expression is abundant in extrahepatic tissues, particularly heart, brain, kidney, skeletal muscle, and lung, but undetectable in liver. It is also detected in leukocytes and fibroblasts.
• Post-translational modifications include phosphorylation, as identified by proteomic studies.
• Mutations in OXCT1 are associated with succinyl-CoA:3-oxoacid CoA transferase (SCOT) deficiency, a disorder of ketone body utilization.
• Key ontological terms include transferase activity, lipid metabolism, and mitochondrial transit peptide processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 261–520 of human OXCT1, a region conserved across species; cross-reactivity with mouse and rat is expected but should be confirmed in relevant sample systems.
• For Western blot, consider using whole-cell lysates from high-expression tissues (e.g., heart, brain) as positive controls. The predicted band is ~56 kDa; validate band appearance under reducing conditions.
• In ELISA applications, the antibody can be used for capture or detection of recombinant OXCT1 protein; optimize coating concentrations and incubation conditions empirically.
• Since OXCT1 is mitochondrial, perform appropriate subcellular fractionation or use mitochondrial markers if localization studies are needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes seamless access to high-quality IVD raw materials. This anti-OXCT1 polyclonal antibody supports ketone metabolism research and assay development. From validated antibody pairs and optimized monoclonals to bulk ancillary reagents and rare target sourcing, we streamline your procurement with a single point of contact—helping you move from concept to clinic efficiently.