OsWRKY53 Rabbit pAb for WB, ELISA - Q5W6M7

Core Product Specifications and Parameters

Biological Background: OsWRKY53 Function and Localization

• OsWRKY53 (WRKY24) is a member of the WRKY transcription factor family in rice (Oryza sativa), characterized by the presence of one or two WRKY domains that mediate sequence-specific DNA binding.
• It functions as a transcriptional activator, regulating gene expression in response to plant hormone signaling pathways, including abscisic acid (ABA) and gibberellin (GA).
• Subcellular localization studies indicate that OsWRKY53 predominantly resides in the nucleus, consistent with its role as a transcription factor.
• The protein contains a conserved WRKY domain repeat, which is essential for recognizing W-box (TTGACC/T) cis-elements in the promoters of target genes.
• Proteomics identification confirms its expression in rice, and it is part of the reference proteome, highlighting its biological significance.
• As a key mediator in ABA and GA signaling, OsWRKY53 likely participates in plant stress responses and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-181 of Oryza sativa OsWRKY53 (Q5W6M7). This N-terminal region may include part of the WRKY domain; consider verifying epitope specificity in your experimental system.
• For Western blot (WB) analysis, a band of approximately 51 kDa is expected based on the predicted molecular weight. Optimize blocking and washing conditions for rice tissue or cell lysates to minimize non-specific binding.
• In ELISA applications, use appropriate positive controls (e.g., recombinant OsWRKY53 protein) and secondary antibody reagents suited for rabbit primary antibodies. Validate assay performance with known standards.
• Given the antibody's application in WB and ELISA, it may be suitable for semi-quantitative protein level assessments; cross-reactivity is reported for Oryza sativa, so ensure sample sourcing matches this specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides a streamlined procurement channel for diagnostic manufacturers and research laboratories worldwide. This OsWRKY53 rabbit polyclonal antibody, targeting a key rice transcription factor, is part of our extensive inventory of validated antibodies for plant and agricultural research. Beyond standard catalog products, CamelBio supports custom sourcing of rare targets, bulk ancillary reagents, and technical consulting to accelerate your assay development from concept to clinic.