Osteopontin (OPN) Monoclonal Antibody for WB, ELISA - P10451

Core Product Specifications and Parameters

Biological Background: Osteopontin Function and Localization

• Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP-1), bone sialoprotein 1, and urinary stone protein, is a secreted glycoprotein encoded by the SPP1 gene.
• It is a major non-collagenous bone matrix protein that binds tightly to hydroxyapatite and is integral to mineralized matrix formation, facilitating cell-matrix interactions.
• OPN functions as a cytokine, enhancing production of interferon-gamma and interleukin-12 while suppressing interleukin-10, thereby promoting type I immunity.
• The protein is secreted and can be detected in bone, plasma, cerebrospinal fluid, and urine.
• Key post-translational modifications include glycosylation, phosphorylation, and sialylation, contributing to its functional diversity.
• Alternative splicing generates multiple isoforms, potentially affecting its tissue-specific roles.
• OPN is classified under keywords like Cell adhesion, Biomineralization, and Cytokine, underscoring its multifaceted biological roles.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole-cell lysates from human, mouse, or rat tissues expressing Osteopontin, such as bone, kidney, or immune cells.
• Given the secreted nature, optimize sample preparation to include secreted forms; consider using serum-free conditioned medium or urine samples for detection.
• For ELISA, the antibody may be suitable as a capture or detection reagent; validate in the relevant sample matrix, with appropriate antigen standards.
• Because Osteopontin is heavily modified post-translationally, the observed molecular weight may differ from the predicted 35 kDa due to glycosylation and phosphorylation.
• Cross-reactivity with mouse and rat enables translational research models; verify reactivity in the relevant species' sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This Osteopontin antibody complements our portfolio of bone metabolism and immunology reagents, where we offer validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets. Connect with us to streamline your procurement and accelerate your assay development.