Osteocalcin (BGLAP) Rabbit mAb for WB, IF/ICC, ELISA - P02818

Core Product Specifications and Parameters

Biological Background: Osteocalcin Function and Localization

• Osteocalcin (BGLAP), also known as Bone Gla protein (BGP), is a small secreted protein belonging to the osteocalcin family.
• The carboxylated form is a major bone matrix component, binding to calcium and hydroxyapatite, and acts as a negative regulator of bone formation. Related references: PMID:3019668, PMID:6967872, PMID:39880952
• Uncarboxylated osteocalcin functions as a hormone, regulating energy metabolism by promoting pancreatic β-cell proliferation, insulin secretion, and sensitivity.
• It plays a role in male fertility by stimulating testosterone production in Leydig cells through the GPRC6A receptor.
• Osteocalcin crosses the blood-brain barrier and acts via GPR158 to support brain development, preventing neuronal apoptosis and modulating neurotransmitter synthesis.
• The protein is secreted and undergoes γ-carboxylation, forming gamma-carboxyglutamic acid residues essential for its calcium-binding activity.
• Additional post-translational modifications include a signal peptide and a disulfide bond. Keywords: Biomineralization, Calcium, Hormone, Secreted.

Experimental Guidance and Technical Tips

• For WB: The antibody detects endogenous Osteocalcin; an expected band around 11 kDa may be observed. Consider using reducing conditions and validate in appropriate cell or tissue lysates.
• For IF/ICC: Fixation and permeabilization steps may be required; consider validating in osteoblast cell lines.
• For ELISA: Can be utilized in sandwich ELISA formats; consider pairing with another anti-Osteocalcin antibody for capture-detection.
• Cross-reactivity with mouse and rat enables translational studies in rodent models.
• Since Osteocalcin is a secreted protein, sample preparation from serum, plasma, or cell culture supernatant may be appropriate for certain applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our Osteocalcin monoclonal antibody supports research in bone metabolism and endocrine signaling, and we offer validated antibody pairs, optimized antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development. Partner with CamelBio to accelerate your diagnostic projects with reliable raw material supply and expert support.