Orexin B Rabbit Monoclonal Antibody for WB, IHC, IF/ICC, ELISA - O43612

Core Product Specifications and Parameters

Biological Background: Orexin B/Hypocretin-2 Function and Localization

• The HCRT gene encodes the prepro-orexin precursor, which is proteolytically processed to produce two mature neuropeptides: orexin-A (hypocretin-1) and orexin-B (hypocretin-2).
• Orexin-B/hypocretin-2 is a selective agonist for the orexin receptor HCRTR2/OX2R, while orexin-A binds both HCRTR1 and HCRTR2 with high affinity.
• Orexin neurons project widely from the lateral hypothalamus, and the peptides are stored in cytoplasmic vesicles at synaptic terminals; subcellularly, the precursor localizes to the rough endoplasmic reticulum.
• Central orexin signaling regulates feeding behavior, energy metabolism, and sleep-wake cycles; dysfunction is linked to sleep disorders such as narcolepsy (UniProt keyword: Disease variant).
• Orexin-B modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
• The precursor undergoes post-translational modifications including amidation, cleavage at paired basic residues, and disulfide bond formation to generate bioactive peptides.
• Abundantly expressed in the subthalamic nucleus; undetectable in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas (hypothalamus not tested in the referenced study).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein of human Orexin B/Hypocretin-2; antibody specificity should be validated in the relevant sample system.
• For Western blot, the predicted molecular weight of the precursor is 13 kDa, but mature peptides are smaller; consider optimizing detection conditions and including appropriate controls.
• For IHC-P, antigen retrieval methods may require empirical optimization; positive control tissues such as subthalamic nucleus are recommended.
• For IF/ICC, co-localization studies with synaptic markers can help confirm localization; background staining should be assessed with isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research institutes, offering a comprehensive portfolio of IVD raw materials, technical services, and consultative support from concept to clinic. This Orexin B monoclonal antibody is a key tool for neuropeptide signaling and sleep disorder research; we can further source validated antibody pairs, recombinant proteins, and bulk ancillary reagents for orexin pathway studies. Contact us for customized sourcing solutions that accelerate your project development.