Oncostatin M Rabbit pAb for WB, IF/ICC, ELISA - P13725

Core Product Specifications and Parameters

Biological Background: Oncostatin M Function and Localization

• Oncostatin M (OSM) is a secreted glycoprotein belonging to the interleukin-6 (IL-6) family of cytokines, characterized by conserved disulfide bonds and cleavage on pair of basic residues.
• It exerts its biological activities through binding to two distinct receptor complexes: the type I receptor (heterodimer of LIFR and IL6ST/gp130) and the type II receptor (heterodimer of OSMR and IL6ST/gp130). Related references: PMID:10997905 PMID:39532904 PMID:8999038
• Upon ligand binding, receptor dimerization activates associated JAK kinases (JAK1, JAK2, TYK2), which phosphorylate receptor intracellular domains, creating docking sites for STAT3 and STAT5B transcription factors. Related references: PMID:9188471 PMID:8999038
• Additionally, the type II receptor recruits SHC1 via OSMR, leading to GRB2 association and activation of the Ras/Raf/MAPK signaling cascade. Related references: PMID:11016927
• OSM regulates diverse biological processes, including cell proliferation, differentiation, and cytokine production, and plays a role in inflammation, hematopoiesis, and tissue remodeling. Related references: PMID:10997905 PMID:1542792 PMID:1542793
• The protein is synthesized as a precursor with a signal peptide and is secreted, where it functions as a mitogen and growth regulator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature Oncostatin M protein (amino acids 26–252), which lacks the signal peptide, likely enabling recognition of the secreted, biologically active form.
• For Western blot (WB), the predicted molecular weight of the unmodified monomer is approximately 28 kDa; however, glycosylation may shift the band, so consider using positive controls expressing OSM.
• This polyclonal antibody is suitable for detecting OSM in human, mouse, and rat samples, but cross-reactivity should be validated for each specific application.
• For IF/ICC, ensure appropriate permeabilization and fixation conditions, as secreted cytokines may require optimized protocols.
• In ELISA, use as a capture or detection antibody after confirming pairing compatibility with available OSM standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, high-quality polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. For cytokine research targeting Oncostatin M, we provide reliable anti-OSM antibodies and can source rare raw materials tailored to your immunoassay needs. Partner with CamelBio to streamline your supply chain and accelerate your assay development.