OGDHL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9ULD0

Core Product Specifications and Parameters

Biological Background: OGDHL Function and Localization

• OGDHL (2-oxoglutarate dehydrogenase-like) is the E1-like component of the 2-oxoglutarate dehydrogenase multienzyme complex (OGDHC), also designated 2-oxoglutarate dehydrogenase complex component E1-like (OGDC-E1-like) or alpha-ketoglutarate dehydrogenase-like.
• In the TCA cycle, it catalyzes the decarboxylation of alpha‑ketoglutarate to succinyl‑CoA and CO₂ while reducing NAD⁺ to NADH. This activity is predominantly mitochondrial.
• OGDHL participates in the inhibition of cell proliferation and in apoptosis. Related references: PMID:23152800 PMID:31175094
• It localizes to the mitochondrial matrix.
• Tissue expression is prominent in the brain and the liver.
• The protein features alternative splicing, calcium‑ and magnesium‑binding sites, and a thiamine pyrophosphate cofactor domain. Mutations are linked to intellectual disability.
• The predicted molecular weight is 114,481 Da, consistent with the observed 114 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–110 (N‑terminal region) of human OGDHL. The antibody is therefore expected to bind the extreme N‑terminus, which should be considered when studying isoforms or cleavage products.
• For western blotting, prepare mitochondrial‑enriched lysates or whole‑cell lysates from brain or liver tissue; the target migrates at approximately 114 kDa. Use 6–10% SDS‑PAGE for high‑molecular‑weight resolution.
• The antibody cross‑reacts with mouse and rat OGDHL. When using rodent samples, validate specificity by testing relevant tissue controls and comparing expected band sizes.
• For IHC‑P, optimize antigen retrieval and antibody dilution empirically on positive control tissues (e.g., human liver or brain sections).
• ELISA performance depends on coating conditions and detection reagents; include a standard curve with recombinant OGDHL protein as reference.
• Shipping on ice bags preserves activity; upon arrival, store aliquots at –20°C or –80°C and avoid repeated freeze‑thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop IVD raw‑material partner, supplying critical reagents such as the OGDHL antibody for metabolism and oncology research programs. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our global sourcing capability extends to rare targets, providing a seamless bridge from early‑stage concept to clinical application.