Occludin Rabbit Polyclonal Antibody for WB and ELISA - Q16625

Core Product Specifications and Parameters

Biological Background: Occludin Function and Localization

• Occludin, encoded by the OCLN gene, is a transmembrane protein localized at tight junctions and belongs to the tight junction family.
• It plays a key role in the formation and regulation of the tight junction paracellular permeability barrier; over-expression induces adhesion in cells lacking tight junctions.
• Occludin acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes, facilitating viral entry.
• Subcellularly, it resides at the cell membrane and is specifically concentrated at tight junctions.
• Highly expressed in kidney, it localizes to tight junctions of both epithelial and endothelial cells but is not detected in testis.
• Post-translationally, Occludin is a phosphoprotein with disulfide bonds; multiple isoforms result from alternative splicing.
• The canonical monomer has a calculated molecular weight of approximately 59 kDa and features coiled-coil domains and transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 350-450; it may recognize total Occludin across the listed species (human, mouse, rat). For Western blot, a band around 59 kDa is expected; validate in the relevant sample system (e.g., kidney lysate).
• For ELISA, consider using this antibody as a capture or detection reagent in a sandwich format; optimize coating and detection conditions for your specific assay design.
• Always include appropriate positive and negative controls, and consider testing multiple dilutions to determine the optimal working concentration for your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a streamlined supply chain for IVD raw materials. This anti-Occludin polyclonal antibody is part of our tight junction and cell permeability portfolio, and we can provide validated antibody pairs, bulk quantities, and ancillary reagents to accelerate your assay development. For rare-target sourcing or custom needs, our team offers integrated consulting and procurement services to bridge the gap from concept to clinical application.