NXN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6DKJ4

Core Product Specifications and Parameters

Biological Background: Nucleoredoxin Function and Localization

• Nucleoredoxin (NXN), also known as NRX, RRS2, or TRG-4, is a 48 kDa redox-active protein belonging to the thioredoxin family. It contains a conserved thioredoxin domain and functions as an NAD-dependent oxidoreductase.
• Acts as a redox-dependent negative regulator of the canonical Wnt signaling pathway. It likely prevents the ubiquitination of Dishevelled-3 (DVL3) by the BCR(KLHL12) E3 ubiquitin ligase complex, thereby stabilizing DVL3 and inhibiting downstream β-catenin signaling.
• May also serve as a transcriptional regulator and a regulator of protein phosphatase 2A (PP2A), as suggested by similarity to other nucleoredoxins.
• Subcellularly, nucleoredoxin is found in both the cytoplasm (cytosol) and the nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing, generating multiple isoforms. It is linked to developmental processes, cell differentiation, and has been associated with disease variants and dwarfism.
• Key biological keywords: Acetylation, Alternative splicing, Cytoplasm, Developmental protein, Differentiation, Disease variant, Dwarfism, NAD, Nucleus, Oxidoreductase, Wnt signaling pathway.

Experimental Guidance and Technical Tips

• For western blotting, expect a predominant band around 48 kDa, but note that alternative splicing may yield isoforms of different sizes; verify in your sample matrix.
• In immunofluorescence/ICC, anticipate cytoplasmic and nuclear staining patterns; optimize fixation and permeabilization protocols to preserve epitope accessibility.
• For ELISA applications, consider using the recombinant immunogen (aa 100–280) as a positive control to establish assay sensitivity and linearity.
• The polyclonal nature may offer broad isoform recognition; however, cross-reactivity with other thioredoxin domain-containing proteins should be ruled out using appropriate knockout or blocking controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For studies on Wnt signaling and redox regulation, we offer scalable supply of high-quality anti-NXN antibodies and related assay components, facilitating seamless development from discovery to clinical application. Reach out to discuss custom sourcing, bulk orders, or technical consultation tailored to your workflow.