NUR77 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P22736

Core Product Specifications and Parameters

Biological Background: NUR77 (NR4A1) Function and Localization

• Orphan nuclear receptor that binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' and 9-cis-retinoic acid outside its ligand-binding domain. Related references: PMID:18690216, PMID:8121493, PMID:9315652
• Participates in energy homeostasis by sequestering the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Related references: PMID:22983157
• Regulates the inflammatory response in macrophages by repressing transcription of genes involved in the citric acid cycle (TCA).
• Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter. Related references: PMID:15466594
• In the cytosol, upon detection of bacterial LPS and mitochondrial DNA released during pyroptosis, promotes non-canonical NLRP3 inflammasome activation.
• May participate in liver regeneration and vascular response to injury.
• Predominantly nuclear, but also detected in cytoplasm, cytosol, and mitochondrion; expressed in fetal muscle, adult liver, brain, and thyroid.
• Subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 200–300 of human NUR77, a region selected for its likely specificity and antigenicity; verify epitope accessibility in your assay.
• For western blotting, a band at approximately 64 kDa is expected; confirm molecular weight markers and optimize blocking conditions.
• In immunofluorescence/ICC, anticipate nuclear and cytoplasmic staining patterns; co-staining with organelle markers may help resolve localization.
• ELISA protocols should include appropriate controls (e.g., antigen-coated wells, negative sera) to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials to support nuclear receptor and inflammation research, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-NUR77 rabbit pAb. Beyond core reagents, we offer bulk ancillary reagents and can assist with sourcing rare targets, ensuring streamlined procurement for diagnostics development. Partner with us for end-to-end material solutions tailored to your research and manufacturing needs.