NUP214 Rabbit Polyclonal Antibody for WB, IP, ELISA - P35658

Core Product Specifications and Parameters

Biological Background: NUP214 Function and Localization

• NUP214 (nucleoporin 214, also known as CAN/Cain) is a 214 kDa protein that is a key component of the nuclear pore complex.
• It is critical for nucleocytoplasmic transport, serving as a docking site for receptor-mediated import of substrates. Related references: PMID:9049309, PMID:31178128, PMID:8108440
• During adenovirus infection, NUP214 is required for capsid disassembly and nuclear entry of the viral genome.
• The protein localizes to the nuclear pore complex within the nucleus.
• Tissue expression is prominent in thymus, spleen, bone marrow, kidney, brain, and testis.
• Post-translationally, NUP214 is modified by acetylation, phosphorylation, and glycosylation; it can also form isopeptide bonds and is a target for UBL conjugation.
• Chromosomal rearrangements involving NUP214 are associated with leukemia, where it acts as a proto-oncogene.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the C-terminal region (aa 1251-1350), which may detect full-length NUP214 and alternative isoforms.
• For western blotting (WB), the predicted molecular weight is ~214 kDa; consider using a low-percentage SDS-PAGE gel and extended transfer times for optimal separation and transfer.
• For immunoprecipitation (IP), validate antibody-enriched samples with target-specific detection (e.g., mass spectrometry or a second antibody).
• For ELISA, test direct or sandwich formats after optimizing coating conditions.
• Always validate the antibody's performance in your specific sample type and experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide IVD manufacturers, CROs, and research labs with end-to-end raw material supply chain solutions. This NUP214 polyclonal antibody exemplifies our capability to deliver high-quality reagents for nuclear pore biology, nucleocytoplasmic transport, and oncology research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio bridges the gap between concept and clinic.