NUP160 Rabbit Polyclonal Antibody for WB, ELISA - Q12769

Core Product Specifications and Parameters

Biological Background: NUP160 Function and Localization

• NUP160 encodes the 160 kDa nucleoporin (Nup160), a key structural scaffold of the nuclear pore complex (NPC).
• Functions as an essential component of the NPC, mediating poly(A)+ RNA export from the nucleus to the cytoplasm.
  Related references: PMID:11564755, PMID:11684705
• Localizes exclusively to the nuclear envelope, embedding within the NPC at the nuclear basket and cytoplasmic fibrils.
• Post-translational modifications include phosphorylation, consistent with dynamic regulation of NPC trafficking.
• Keywords such as 'mRNA transport', 'Protein transport', and 'Translocation' underscore its role in nucleocytoplasmic shuttling.
• The gene is classified under the 'Disease variant' keyword in UniProt, indicating that mutations in NUP160 are associated with human disorders.
• Alternative splicing produces multiple isoforms, and the protein is a member of the Nup107–160 subcomplex within the NPC.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 40–150) of human NUP160; antibody reactivity may be limited to epitopes within this region and may exhibit species specificity for human.
• For Western blot, anticipate a band near 162 kDa; nuclear-enriched extracts are recommended as positive controls, with careful marker verification.
• Nuclear fractionation or enrichment protocols can improve detection sensitivity in WB due to the exclusive nuclear localization of NUP160.
• In ELISA, validate reactivity using recombinant NUP160 protein and exclude cross-reactivity with other nucleoporins experimentally.
• Suitable for studying nucleocytoplasmic transport mechanisms, but functional assays (e.g., RNA export) require independent validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material sourcing partner, offering validated antibodies, optimized reagent pairs, and bulk auxiliary materials to streamline diagnostic and research workflows from concept to clinic. This anti-NUP160 polyclonal antibody supports targeted investigations in nuclear transport and cell biology, reflecting our broad portfolio for niche intracellular targets. For rare target sourcing, custom scale-up, or tailored technical consultations, reach out to our team to secure reliable, high-quality reagents efficiently.