NUP153 Rabbit Polyclonal Antibody for WB and ELISA - P49790

Core Product Specifications and Parameters

Biological Background: NUP153 Function and Localization

• NUP153 (nucleoporin 153) is a component of the nuclear pore complex (NPC), essential for nucleocytoplasmic transport of proteins and mRNAs.
• It functions as a scaffold in the nuclear phase of the NPC, mediating the anchoring of TPR to the nuclear membrane.
• Involved in quality control and retention of unspliced mRNAs in the nucleus; regulates nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner.
• Contains a repeat domain that may anchor other NPC components to the pore membrane; possible DNA-binding subunit.
• Interacts with HIV-1 capsid protein P24, promoting viral integration in non-dividing cells; binds HIV-2 protein vpx to facilitate nuclear translocation of the lentiviral genome.
• Subcellularly localized to the nucleus, nuclear membrane, and nuclear pore complex.
• Undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination; contains zinc-finger motifs.
• Alternative splicing produces multiple isoforms; the canonical protein has a calculated molecular weight of approximately 154 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering residues 350-450 of human NUP153; consider epitope accessibility and sequence conservation across species when designing experiments.
• For Western blot, the expected band is ~154 kDa; use nuclear-enriched lysates from human, mouse, or rat cells as positive controls. Validate antibody specificity with appropriate blocking peptides or knockout controls.
• In ELISA, optimize coating antigen and antibody dilution to ensure linear detection range; cross-reactivity with mouse and rat NUP153 may require species-specific standard curves.
• As a polyclonal antibody, it may recognize multiple isoforms or degradation products; confirm band patterns in your sample system.
• The antibody is shipped on ice bags; upon receipt, store at -20°C or as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined path to IVD raw-material procurement, covering the entire development cycle from concept to clinic. With this NUP153 polyclonal antibody, researchers gain a reliable tool for studying nuclear transport mechanisms and host-pathogen interactions. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assists in sourcing rare-target raw materials to accelerate your assay development and production scale-up.