NUP133 Rabbit pAb - Q8WUM0

Core Product Specifications and Parameters

Biological Background: NUP133 Function and Localization

• NUP133 encodes a 133 kDa nucleoporin that serves as a core structural component of the nuclear pore complex (NPC), mediating bidirectional nucleocytoplasmic transport.
• It is directly involved in the export of poly(A)+ RNA from the nucleus to the cytoplasm, facilitating mRNA trafficking.
• NUP133 plays a critical role in nephrogenesis, contributing to proper kidney development. Related references: PMID:30179222
• Subcellularly, NUP133 localizes primarily to the nuclear pore complex and also associates with the chromosome centromere and kinetochore during mitosis.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which regulate its dynamic interactions and functions.
• The protein is widely expressed in both fetal and adult tissues, with prominent expression observed in the brain and kidney.
• Mutations in NUP133 are linked to disease phenotypes such as epilepsy and intellectual disability, underscoring its importance in cellular and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 730-930, a central region of human NUP133; consider validating reactivity with full-length protein in the appropriate sample system.
• For Western blotting, owing to the 129 kDa molecular weight, use a low-percentage polyacrylamide gel (e.g., 8%) and optimize transfer conditions to ensure efficient detection.
• The polyclonal nature may provide robust signal amplification; however, consider including a blocking step and secondary antibody controls to minimize non-specific binding.

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