Nup107 Rabbit mAb - P57740

Core Product Specifications and Parameters

Biological Background: Nup107 Function and Localization

• Nup107 is a core component of the nuclear pore complex (NPC), a massive protein assembly that governs nucleocytoplasmic transport.
• It plays an essential role in NPC assembly and/or maintenance, as studies demonstrate its requirement for the incorporation of peripheral proteins into the NPC (PubMed:12552102, PubMed:15229283, PubMed:30179222). Related references: PMID:12552102, PMID:15229283, PMID:30179222
• Nup107 may anchor the nucleoporin NUP62 to the NPC, contributing to structural stability (PubMed:15229283). Related references: PMID:15229283
• Beyond its structural role, Nup107 is involved in nephrogenesis, with mutations linked to kidney developmental defects (PubMed:30179222). Related references: PMID:30179222
• Subcellularly, Nup107 localizes to the nuclear membrane and NPC, and also associates with chromosome centromeres and kinetochores during mitosis, highlighting its dual function in interphase and cell division.
• The protein is ubiquitously expressed across fetal and adult tissues, reflecting its fundamental role in cellular physiology.
• Post-translational modifications of Nup107 include acetylation, phosphorylation, and methylation, which may modulate its function and protein-protein interactions.
• Alternative splicing produces multiple isoforms, and variants in NUP107 have been associated with diseases such as steroid-resistant nephrotic syndrome, Galloway-Mowat syndrome, epilepsy, and intellectual disability.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 700-800 within the C-terminal region of human Nup107. For Western blotting, anticipate a specific band near 106 kDa; validate sensitivity and specificity using human cell lysates and appropriate controls (e.g., siRNA knockdown or overexpression).
• In ELISA formats, the peptide immunogen may facilitate direct detection; optimize coating and blocking conditions, and confirm linearity and sensitivity with recombinant Nup107 or peptide standards.
• Reported cross-reactivity is with human Nup107; for non-human species, assess sequence homology and test experimentally before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD manufacturers, research laboratories, and diagnostic developers with a streamlined one-stop supply chain for raw materials like this Nup107 monoclonal antibody. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated support for sourcing even the most niche target raw materials. From nuclear pore complex studies to nephrology-focused assay development, CamelBio accelerates your path from concept to clinic.