NTN5 Rabbit Polyclonal Antibody for WB and ELISA - Q8WTR8

Core Product Specifications and Parameters

Biological Background: NTN5 Function and Localization

• NTN5 encodes netrin-5, a secreted protein belonging to the netrin family, also known as netrin-1-like protein.
• It plays a critical role in neurogenesis by preventing motor neuron cell body migration out of the neural tube, ensuring proper neural development.
• The protein contains laminin EGF-like domain repeats, characteristic of the netrin family, which mediate interactions with receptors.
• NTN5 is a secreted glycoprotein with a signal peptide and disulfide bonds, indicating post-translational modifications essential for its structure and function.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional roles in development.
• Netrin-5 is classified as a developmental protein and is involved in axon guidance and cell migration processes.
• The protein has a molecular weight of approximately 53 kDa, consistent with its glycosylated form.

Experimental Guidance and Technical Tips

• For WB, an expected band at ~53 kDa may be observed; validate detection in your specific tissue or cell lysate system, considering the antibody's polyclonal nature.
• Due to the secreted nature of NTN5, analyzing cell culture supernatants or extracellular matrix fractions could enhance detection sensitivity.
• When performing ELISA, include appropriate controls to confirm specificity, especially when comparing samples across human, mouse, and rat.
• Consider reducing and non-reducing SDS-PAGE conditions to evaluate disulfide bond influences on epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This NTN5 antibody supports neurobiology and developmental biology research areas, and we can assist in sourcing additional rare-target reagents or custom antibody development to meet your specific assay requirements. Let CamelBio be your partner from concept to clinic.