NTAN1 Rabbit Polyclonal Antibody for WB and ELISA - Q96AB6

Core Product Specifications and Parameters

Biological Background: NTAN1 Function and Localization

• NTAN1 (also known as PNAA or PNAD) is a protein N-terminal asparagine amidohydrolase that catalyzes the deamidation of N-terminal asparagine residues to aspartate.
• This enzyme is essential for the N-end rule pathway, enabling the ubiquitin-dependent degradation of proteins that start with Met-Asn by facilitating subsequent arginylation and polyubiquitination.
• NTAN1 specifically acts on proteins with an exposed N-terminal asparagine after removal of the initiator methionine; it does not modify internal or C-terminal asparagine residues or any glutamine residues.
• The protein localizes to the cytoplasm, consistent with its role in cytosolic protein quality control.
• NTAN1 is a member of the hydrolase family and has a reference proteome status; its three-dimensional structure has been experimentally determined.
• It contributes to proteostasis by regulating the turnover of proteins with destabilizing N-terminal residues.

Experimental Guidance and Technical Tips

• The immunogen spans almost the full-length NTAN1 protein (residues 1–310), which may result in recognition of multiple epitopes and could be suitable for detecting endogenous NTAN1 in various sample types.
• When performing Western blotting, a band around 35 kDa is predicted; consider using appropriate positive controls (e.g., human, mouse, or rat cell lysates) and validate detection in your specific experimental system.
• The reported cross-reactivity with human, mouse, and rat suggests conserved epitopes, making this antibody a potential tool for cross-species studies; however, verify performance in your target species.
• For ELISA, the antibody may be used as a detection reagent; optimization of coating antigen concentration and antibody dilution is recommended to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined sourcing solution for IVD raw materials, including this NTAN1 antibody for investigations into the N-end rule pathway and protein degradation. Our one-stop platform offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare targets, helping you accelerate development from concept to clinic.