NRN1L Rabbit pAb for WB, ELISA - Q496H8

Core Product Specifications and Parameters

Biological Background: NRN1L Function and Localization

• NRN1L, also known as UNQ2446, cpg15-2, or MRCC2446, is a neuritin-like protein encoded by the NRN1L gene in humans.
• The protein is predicted to contain a signal peptide and a GPI-anchor, classifying it as a membrane-bound glycoprotein.
• It localizes to the cell membrane, as annotated in UniProt (Q496H8).
• The molecular weight of the unprocessed precursor is approximately 17.8 kDa, consistent with the 18 kDa band typically detected in Western blots.
• NRN1L is designated as a reference proteome constituent and has been identified through proteomics identification approaches.
• Key structural features include a signal sequence for secretion, a glycoprotein domain, and a GPI-anchor for membrane attachment.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is ~18 kDa; consider using reducing SDS-PAGE and standard transfer conditions.
• In ELISA, the antibody can be employed for antigen capture or detection; validate with recombinant protein or relevant cell lysates.
• Cross-reactivity with mouse and rat indicates conserved epitopes; the antibody may be suitable for rodent model studies.
• As the immunogen covers amino acids 39-139, the antibody likely recognizes the mature extracellular domain after signal peptide cleavage.
• Being a polyclonal antibody, it may detect multiple epitopes; affinity purification could be considered for applications requiring high specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing solution for IVD raw materials, including validated antibodies like this NRN1L Rabbit pAb. Our portfolio covers a broad range of rare targets and ancillary reagents to support your neuroscience and cell biology assay development. From antibody pairs to bulk raw materials, we streamline procurement from concept to clinic.