NR5A2 Rabbit pAb for WB, IF-P, IHC-P, ELISA - O00482

Core Product Specifications and Parameters

Biological Background: NR5A2 Function and Localization

• NR5A2 (also known as LRH-1, B1F, CPF, FTF) is an orphan nuclear receptor that binds DNA as a monomer to the 5'-TCAAGGCCA-3' sequence and regulates transcription of target genes involved in development, metabolism, and inflammation. Related references: PMID:16289203, PMID:18410128, PMID:21614002

• Acts as a pioneer transcription factor, unwrapping target DNA from histones and eliciting local opening of closed chromatin, essential for early embryonic development. Related references: PMID:38409506

• Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver, controlling bile acid synthesis. Related references: PMID:10359768

• Involved in lipid metabolism, negative regulation of inflammation in liver and pancreas, and protection against intestinal inflammation via glucocorticoid production regulation. Related references: PMID:20159957

• Binds phospholipid ligands in vitro, such as phosphatidylethanolamine, which induce conformational change and coactivator recruitment. Related references: PMID:15707893, PMID:15723037, PMID:15897460

• Localized to the nucleus and associated with chromosomes.

• Plays a role in hepatitis B virus gene transcription and DNA replication by cooperatively up-regulating enhancer II activity with HNF1A.

• Abundantly expressed in pancreas, less in liver; isoform 1 and isoform 2 present in fetal and adult liver and Hep-G2 cells. Related references: PMID:9786908

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 300–541), which includes the ligand-binding domain. Consider this when assessing isoform specificity or potential interference from endogenous ligands.
• For western blot, validate detection of the 61 kDa band in appropriate positive controls (e.g., liver, pancreas, or Hep-G2 cells).
• For immunofluorescence or immunohistochemistry, nuclear localization is expected; optimize fixation and permeabilization conditions for your sample type.
• The antibody’s cross-reactivity with mouse and rat makes it suitable for cross-species comparative studies; validate in your specific model system.

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