NPY Rabbit mAb for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA - P01303

Core Product Specifications and Parameters

Biological Background: NPY Function and Localization

• Neuropeptide Y (NPY), also known as PYY4, is a 36-amino acid peptide of the pancreatic polypeptide family, encoded by the NPY gene (GeneID: 4852). It is one of the most abundant and widely distributed neuropeptides in the mammalian nervous system.
• NPY acts as a high-affinity ligand for the G protein-coupled receptors NPY1R and NPY2R, triggering adenylate cyclase inhibition and a rise in intracellular Ca²⁺ levels (PubMed:1321422, PubMed:7592910, PubMed:35165283, PubMed:38882210). Related references: PMID:1321422 PMID:7592910 PMID:35165283 PMID:38882210
• Through this signaling, NPY centrally stimulates food intake and regulates energy homeostasis, and peripherally modulates colonic ion secretion and smooth muscle contractility.
• NPY is predominantly localized to secretory vesicles, especially large dense-core vesicles in neurons, and is released upon stimulation to act as a neuromodulator and hormone. It is also found in adrenal medullary chromaffin cells.
• The NPY preproprotein undergoes proteolytic cleavage at dibasic sites and C-terminal amidation to generate the mature, biologically active peptide.
• Keywords for NPY include neuropeptide, amidation, cleavage on pair of basic residues, phosphoprotein, cytoplasmic vesicle, and secreted, reflecting its synthesis, processing, and release pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human NPY, so the antibody likely recognizes a linear epitope. It is expected to perform well in denaturing applications such as Western blotting.
• For WB, use brain, hypothalamus, or gut lysates. NPY is a small peptide (approx. 11 kDa); ensure the use of low-percentage or gradient gels and appropriate protein standards. Consider adding protease inhibitors to prevent degradation.
• For immunofluorescence (IF) and immunohistochemistry (IHC), antigen retrieval may enhance signal. Fixation methods (e.g., 4% PFA) are compatible, but validate in your specific tissue because NPY is a secreted and rapidly degraded peptide.
• In ELISA, the antibody can be used for capture or detection, but pairing with another NPY antibody for sandwich ELISA requires empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated supply solutions for IVD manufacturers and research labs, providing a comprehensive range of raw materials from antibody pairs to bulk ancillary reagents. This anti-NPY monoclonal antibody supports studies in neurobiology, metabolism, and gastrointestinal disorders, aligning with CamelBio's capability to source rare-target reagents and deliver quality-controlled products that accelerate assay development. With one-stop access to validated antibodies and technical consultation, CamelBio simplifies procurement for diagnostic and research workflows.