NPPA Rabbit pAb - P01160

Core Product Specifications and Parameters

Biological Background: NPPA Function and Localization

• NPPA encodes natriuretic peptides A, a hormone critical for cardio-renal homeostasis, vascular remodeling, and energy metabolism regulation. Related references: PMID:15741263, PMID:16875975, PMID:18835931
• Signals through NPR1 receptor to elevate cGMP, activating kinases like PRKG1 to mediate vasodilation, natriuresis, and diuresis. Related references: PMID:1660465, PMID:1672777, PMID:21098034
• Essential for blood pressure control and fluid-electrolyte balance by regulating aldosterone synthesis. Related references: PMID:2532366, PMID:2825692, PMID:7595132
• Inhibits cardiac remodeling and hypertrophy by promoting cardiomyocyte apoptosis and attenuating growth. Related references: PMID:16875975
• In adipose tissue, modulates lipid metabolism and energy expenditure via cGMP/PKG and AMPK pathways. Related references: PMID:15741263, PMID:18835931, PMID:21672517
• Binds clearance receptor NPR3 for removal from circulation. Related references: PMID:1672777
• The urodilatin form, produced in kidneys, specifically regulates renal sodium and water homeostasis. Related references: PMID:8351194, PMID:8779891, PMID:8853410, PMID:8384600, PMID:9893117
• Secreted protein; predominantly detected in atrial and ventricular plasma, adipocytes, brain astrocytes, and kidney distal tubular cells.

Experimental Guidance and Technical Tips

• The immunogen spans a large portion of the NPPA preproprotein, so the antibody may recognize both unprocessed and cleaved forms; verify band patterns in relevant samples.
• For Western blot, a predicted band around 16 kDa is expected for the unprocessed protein, but mature ANP is only ~3 kDa; consider using a 15% gel or gradient for low molecular weight proteins.
• Validate the antibody in IF-P on cardiac or renal tissue sections where NPPA is highly expressed; use appropriate fixation and permeabilization.
• ELISA performance should be confirmed with recombinant NPPA or relevant biological fluids; include proper controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated IVD raw materials including polyclonal antibodies like this NPPA pAb, tailored for cardiovascular and renal diagnostics development. We offer one-stop procurement of antibody pairs, ancillary reagents, and rare target sourcing to streamline your assay pipeline from concept to clinic. Contact us for bulk orders and technical consultation.