NPM1 (B23, NPM) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06748

Core Product Specifications and Parameters

Biological Background: NPM1 Function and Localization

• Nucleophosmin (NPM1, also known as B23, NO38, or numatrin) is a multifunctional nucleolar phosphoprotein involved in ribosome biogenesis, centrosome duplication, histone assembly, and tumor suppressor regulation.
• Acts as a histone chaperone for core histones H3, H2B, and H4, facilitating nucleosome assembly.
• Binds ribosomes and single-stranded nucleic acids, likely driving nuclear export of ribosomal subunits.
• Regulates centrosome duplication in concert with BRCA2 and controls centriole replication via PLK2-mediated phosphorylation.
• Antagonizes ATF5-induced G2/M blockade, promoting cell proliferation.
  Related references:
  PMID:22528486
• In complex with MYC, enhances transcription of MYC target genes.
  Related references:
  PMID:25956029
• Stimulates APEX1 endonuclease activity on AP double-stranded DNA but inhibits it on AP single-stranded RNA, regulating DNA repair and rRNA quality control.
• Subcellular localization includes nucleolus, nucleoplasm, cytoplasm, and centrosome; undergoes acetylation, ADP-ribosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western blotting, NPM1 typically migrates at ~33 kDa; validate the antibody in your specific sample system using appropriate positive and negative controls.
• Since the immunogen spans amino acids 9–122, the antibody may detect multiple isoforms or cleavage products; confirm specificity via peptide competition or knockdown experiments.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilution; expect nuclear/nucleolar staining in accordance with NPM1 localization.
• This polyclonal antibody cross-reacts with mouse and rat NPM1; verify performance in these species before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted provider of IVD raw materials, offering one-stop access to validated antibodies, antibody pairs, and ancillary reagents for diagnostic development. This NPM1 polyclonal antibody supports cancer and cell biology research, and we can further assist with custom monoclonal development or bulk sourcing needs. Let CamelBio bridge the gap from concept to clinic with reliable, high-quality immunoreagents.