NPHP3 Rabbit Polyclonal Antibody for WB and ELISA - Q7Z494

Core Product Specifications and Parameters

Biological Background: NPHP3 Function and Localization

• NPHP3 (Nephrocystin-3) is a protein that contains TPR repeats and coiled coil domains, functioning as a key regulator in ciliary biology and Wnt signaling pathways.
• It localizes specifically to the cilium, a microtubule-based organelle, where it is essential for normal ciliary development and function.
• NPHP3 inhibits the canonical Wnt signaling cascade by suppressing Disheveled-1 activity, and may also modulate non-canonical Wnt/planar cell polarity pathways, acting as a molecular switch between these pathways.
• The protein is required for convergent extension cell movements during development, a process mediated by non-canonical Wnt signaling.
• NPHP3 is widely expressed at low levels, with relatively higher expression in heart, placenta, liver, skeletal muscle, kidney, and pancreas, and very low in brain and lung.
• Defects in NPHP3 are associated with ciliopathies including nephronophthisis, Meckel syndrome, and renal-hepatic-pancreatic dysplasia, highlighting its critical role in organogenesis.

Experimental Guidance and Technical Tips

• For WB, consider using whole cell lysates from tissues such as kidney or heart where NPHP3 expression is relatively higher. Validate the antibody in your specific sample system, as protein expression may be low.
• Since NPHP3 is a large protein (~151 kDa), use appropriate gel percentage and transfer conditions to ensure efficient blotting.
• For ELISA, the recombinant immunogen (aa1-130) can serve as a positive control; titrate the antibody to optimize signal-to-noise ratio.
• Although the antibody is validated for human and mouse, cross-reactivity with other species should be verified experimentally due to possible sequence divergence in the N-terminal region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This NPHP3 polyclonal antibody supports research in ciliopathy and Wnt signaling, and our team can assist with customization and scaling to meet your diagnostic development needs. From early-stage discovery to clinical validation, CamelBio streamlines procurement to accelerate your assay development journey.