NPHP1 Rabbit Monoclonal Antibody for WB and ELISA - O15259

Core Product Specifications and Parameters

Biological Background: Nephrocystin-1 Function and Localization

• Nephrocystin-1 (NPHP1) is encoded by the NPHP1 gene and is also known as juvenile nephronophthisis 1 protein.
• It plays a role in epithelial cell polarity and the organization of apical junctions in kidney cells, interacting with proteins such as BCAR1, NPHP4, and RPGRIP1L.
• NPHP1 is involved in the regulation of intraflagellar transport (IFT) during cilia assembly, influencing the movement of IFT proteins like IFT88 and WDR19 in photoreceptor cilia.
• It localizes to cell junctions, including tight junctions and adherens junctions, and is found within the cilium axoneme and cytoplasm.
• NPHP1 is required for normal retina development and spermatogenesis.
• Tissue expression is widespread, with highest levels in pituitary gland, spinal cord, thyroid, testis, skeletal muscle, lymph node, and trachea; lower in heart, kidney, and pancreas. Related references: PMID:16308564 PMID:18477472
• Associated with ciliopathies such as nephronophthisis, Joubert syndrome, Leber congenital amaurosis, and Senior-Loken syndrome; contains an SH3 domain. Molecular weight predicted at 83 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 463-732 of human NPHP1 (NP_997064.2). This region may confer specificity; verify with sequence alignment if cross-reactivity with other species or isoforms is expected.
• For Western blot, the predicted molecular weight is ~83 kDa; consider using appropriate gel percentage and loading controls.
• ELISA may be used for quantitative detection; validate with appropriate standard curves in the relevant sample system.
• Due to cross-reactivity with mouse and rat, this antibody can be used in rodent models for nephronophthisis or ciliopathy studies.
• Consider optimizing antibody dilution and incubation conditions for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For NPHP1 research and ciliopathy-related applications, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply chain ensures reliable access to high-quality reagents for your assay development and diagnostic needs.