NOXA1 Rabbit pAb for WB and ELISA - Q86UR1

Core Product Specifications and Parameters

Biological Background: NOXA1 Function and Localization

• NOXA1 (NADPH oxidase activator 1), also designated as p51-nox, NY-CO-31, SDCCAG31, or NCF2-like protein, is a member of the p47phox/p67phox family and functions as an activator of the superoxide-generating NADPH oxidase NOX1.
• It plays a central role in the production of reactive oxygen species (ROS) which are involved in diverse biological processes including host defense against pathogens, hormone biosynthesis, oxygen sensing, and intracellular signal transduction.
• NOXA1 is also capable of activating other NADPH oxidase members such as CYBB/gp91phox and NOX3, indicating a broader regulatory role within the NOX family.
• The protein features functional domains including SH3 domains and TPR repeats, and is subject to post-translational modifications such as phosphorylation, as well as alternative splicing, contributing to its functional versatility.
• Subcellularly, NOXA1 is found in both the cytoplasm and the cell membrane, enabling dynamic recruitment and interaction with membrane-bound NOX enzymes.
• It exhibits wide tissue distribution, with notable expression in pancreas, liver, kidney, spleen, prostate, small intestine, and colon, suggesting a ubiquitous role in cellular redox regulation.
• With a calculated molecular mass of approximately 50.9 kDa (observed at 51 kDa), NOXA1 is part of the reference proteome and has been identified in multiple proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 140-300) of human NOXA1, which may enable recognition of full-length protein and select isoforms; verify isoform coverage if your model expresses splice variants.
• For Western blotting, consider testing a concentration gradient of the antibody and validate performance using positive lysates from human, mouse, or rat tissues known to express NOXA1 (e.g., liver, pancreas, or colon).
• In ELISA applications, employ appropriate antigen standards and include negative controls to ensure specificity; optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with reliable, end-to-end access to IVD raw materials, including validated antibodies for targets like NOXA1 in NADPH oxidase/ROS research. From this polyclonal antibody to optimized monoclonal reagents, antibody pair screening, and bulk ancillary reagent supply, we bridge sourcing gaps for both common and rare targets. Our technical support and supply chain solutions help streamline your workflow from concept to clinical application.