NOX5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96PH1

Core Product Specifications and Parameters

Biological Background: NOX5 Function and Localization

• NOX5 is a calcium-dependent NADPH oxidase that generates superoxide (O2•−) from molecular oxygen using NADPH as an electron donor, contributing to reactive oxygen species (ROS) signaling. Related references: PMID:11483596, PMID:14982937, PMID:17275676, PMID:12686516
• An isoform lacking calcium-binding EF-hand domains exhibits calcium-independent oxidase activity, though its activity is debated; it may undergo rapid degradation. Related references: PMID:17275676, PMID:36653838, PMID:22427510
• In endothelial cells, NOX5 mediates thrombin-induced ROS production, proliferation, and angiogenesis, highlighting its role in vascular biology. Related references: PMID:17275676
• NOX5 also synthesizes the calcium-mobilizing second messenger NAADP from NAADPH, impacting T cell activation. Related references: PMID:34784249
• Subcellularly, NOX5 localizes to the endoplasmic reticulum and cell membrane.
• It is primarily expressed in testis (pachytene spermatocytes), spleen, lymph nodes, and endothelial cells, with additional expression in ovary, placenta, pancreas, and cardiac fibroblasts.
• The protein contains FAD- and calcium-binding domains, characteristic of the NADPH oxidase family, and features transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 661–765 (C-terminal region) of human NOX5; the antibody is expected to recognize this region. Consider validating specificity using overexpression or knockdown systems.
• For Western blot, anticipate a band near 86 kDa; additional bands may appear due to isoforms or post-translational modifications; validate in the relevant sample system.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization methods as NOX5 localizes to both endoplasmic reticulum and plasma membrane.
• For ELISA, the antibody may be used for detection; appropriate positive and negative controls are recommended.
• Cross-reactivity has been reported for human only; verify reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonals/polyclonals, and bulk ancillary reagents. This anti-NOX5 polyclonal antibody supports research in oxidative stress, cardiovascular disease, and immunology, and CamelBio can facilitate procurement of rare-target raw materials and technical consultation for your development pipeline.