NOTCH3 Rabbit pAb - Q9UM47

Core Product Specifications and Parameters

Biological Background: NOTCH3 Function and Localization

• NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
  Related references:
  PMID:15350543 PMID:14714274
• Upon ligand activation, the notch intracellular domain (NICD) is released and forms a transcriptional activator complex with RBPJ/RBPSUH to activate genes of the enhancer of split locus.
• NOTCH3 signaling affects the implementation of differentiation, proliferation and apoptotic programs.
• The protein is localized to the cell membrane and translocates to the nucleus upon activation.
• NOTCH3 is ubiquitously expressed in fetal and adult tissues.
• It contains EGF-like domains and ANK repeats; it is involved in the Notch signaling pathway, and mutations are associated with the disease CADASIL.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1520-1640 of human NOTCH3. Consider validating antibody specificity using full-length protein or relevant cell lysates.
• For Western blot, note the predicted molecular weight of approximately 244 kDa; use appropriate gel percentage and transfer conditions to ensure proper separation and detection.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization to detect both membrane and nuclear localization.
• For ELISA, ensure the coating antigen correctly presents the epitope region.
• When using across species (human, mouse, rat), confirm cross-reactivity with positive and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, from concept to clinic. This NOTCH3 polyclonal antibody supports research in developmental biology and CADASIL disease mechanisms. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets to accelerate your diagnostic and research workflows.