NOP58 Rabbit pAb for WB, IF/ICC, ELISA - Q9Y2X3

Core Product Specifications and Parameters

Biological Background: NOP58 Function and Localization

• NOP58 (nucleolar protein 5, NOL5/NOP5) is a core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that methylate multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs). Related references: PMID:39570315
• NOP58 is required for the biogenesis of box C/D snoRNAs (e.g., U3, U8, U14) and functions as part of the small subunit (SSU) processome during early ribosomal subunit assembly, promoting pre-rRNA folding, modifications, cleavage, and exosome-mediated degradation. Related references: PMID:15574333, PMID:17636026, PMID:19620283, PMID:34516797
• NOP58 localizes predominantly to the nucleolus and nucleoplasm.
• It is expressed ubiquitously across human tissues.
• The protein undergoes phosphorylation and isopeptide bond formation, and is targeted by ubiquitin conjugation; 3D structural data are available.
• NOP58 is a critical ribonucleoprotein factor in ribosome biogenesis, with roles in RNA modification and quality control.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 360–529) of human NOP58; verify specificity in your model system by knockdown or knockout controls.
• For Western blotting, expect a band at approximately 60 kDa; optimize antibody dilution using a titration series.
• In immunofluorescence / ICC, consider antigen retrieval and permeabilization protocols suitable for nucleolar targets; co-stain with nucleolar markers to confirm localization.
• Cross-reactivity is reported for human, mouse, and rat; if working with other species, pilot testing is advisable.
• As a polyclonal antibody, performance may vary between lots; include appropriate positive and negative controls and re-validate for critical assays.
• For ELISA, test as a capture or detection reagent with a matched pair approach for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your ribosome biogenesis and nucleolar research by supplying validated anti-NOP58 polyclonal antibodies together with end-to-end raw material sourcing. From optimized monoclonal/polyclonal antibody pairs and bulk ancillary reagents to rare-target development, our platform connects diagnostic manufacturers and labs with the critical IVD raw materials needed to move from concept to clinic.