nm23 Rabbit Polyclonal Antibody for IHC-P and ELISA - P15531 / P22392

Core Product Specifications and Parameters

Biological Background: nm23 Function and Localization

• The nm23 antibody recognizes both human NME1 (P15531) and NME2 (P22392), also known as NM23-H1 and NM23-H2, respectively. NME1 is a nucleoside diphosphate kinase that catalyzes the transfer of a γ-phosphoryl group from ATP to nucleoside diphosphates, maintaining nucleotide homeostasis. Related references: PMID:10952986 PMID:14960567 PMID:16313181
• Beyond its kinase activity, NME1 functions as a histidine protein kinase and a non-specific serine/threonine kinase, phosphorylating target proteins. Related references: PMID:8529641 PMID:9038158
• NME1 also phosphorylates geranyl and farnesyl pyrophosphate, connecting nucleotide metabolism with isoprenoid synthesis. Related references: PMID:10952986
• As a component of the SET complex, NME1 exhibits Mg²⁺-dependent single-stranded DNA endonuclease activity, cooperating with TREX1 to promote apoptotic DNA fragmentation in immune cells. Related references: PMID:12628186 PMID:16818237
• NME1 can cleave double-stranded DNA at specific promoter regions, such as the PDGFA gene, potentially regulating gene expression. Related references: PMID:11694515
• It may also act as a 3′-5′ DNA exonuclease, excising nucleotides from single-stranded DNA or 3′ overhangs, suggesting roles in DNA nucleolytic processing. Related references: PMID:14960567 PMID:16313181
• NME1 is key regulator of tumor metastasis and cellular differentiation, and is required for cell motility. Related references: PMID:8270257 PMID:25582197
• Subcellular localization includes cytoplasm, nucleus, and cell membrane; the protein is ubiquitously expressed and highly detectable in tumor cell lines.
• Post-translational modifications such as acetylation, phosphorylation, and glutathionylation have been reported.

Experimental Guidance and Technical Tips

• As a rabbit polyclonal antibody raised against a recombinant protein spanning amino acids 1–152 of both NME1 and NME2, this product likely detects both isoforms in their native and denatured forms. For IHC-P, consider using antigen retrieval with citrate buffer (pH 6.0) and a standard ABC/DAB detection system; include appropriate positive and negative tissue controls (e.g., tumor vs. normal tissue). For ELISA, employ an indirect format with the antibody as the detection reagent or capture, expecting a linear dose-response within the recommended working dilution range. Note that polyclonal antibodies may exhibit batch-to-batch variability; we recommend titrating each new lot to optimize performance. While not validated for Western blot, a band at ~17 kDa may be observed under reducing conditions; however, users should perform their own validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the path from concept to clinic by providing diagnostic manufacturers, research laboratories, and institutes with one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consulting. This high-quality nm23 polyclonal antibody exemplifies our commitment to supplying optimized reagents for nucleoside diphosphate kinase and metastasis research. Whether you need validated antibody pairs, bulk ancillary reagents, or support sourcing rare-target raw materials, CamelBio is your trusted partner in accelerating assay development and manufacturing.