NLRX1 Rabbit pAb - Q86UT6

Core Product Specifications and Parameters

Biological Background: NLRX1 Function and Localization

• NLRX1 (NOD5, NOD9, NOD26, DLNB26, CLR11.3) is a member of the NLR (nucleotide-binding domain and leucine-rich repeat containing) family, characterized by an N-terminal mitochondrial targeting sequence (transit peptide), a central NACHT domain with ATP-binding activity, and C-terminal leucine-rich repeats (LRRs).
• Negatively regulates the virus-induced RLH-MAVS interaction, thereby inhibiting MAVS-mediated antiviral signaling (PubMed:18200010). Related references: PMID:18200010
• Promotes autophagy by interacting with TUFM and subsequently recruiting ATG5 and ATG12 (PubMed:22749352). Related references: PMID:22749352
• Regulates MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis (PubMed:27393910). Related references: PMID:27393910
• Enhances NF-kappa-B and JNK signaling through the production of reactive oxygen species (PubMed:18219313). Related references: PMID:18219313
• Exhibits sex-dependent regulation of viral-mediated inflammation and energy metabolism, potentially contributing to sex differences observed in infectious diseases (By similarity).
• Localizes to the mitochondrial outer membrane.
• Ubiquitously expressed, with strongest levels in mammary gland, heart, and muscle; detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines (at protein level).

Experimental Guidance and Technical Tips

• For Western blot, target protein runs at approximately 108kDa; consider verifying band specificity with knockdown controls and using appropriate protein ladders.
• In immunoprecipitation, test antibody suitability with endogenous NLRX1 from relevant cell lines (e.g., HeLa, HEK293T) known to express the protein.
• The immunogen corresponds to a recombinant fragment (aa 87-360); multiple isoforms or splice variants may be detected; validate in your experimental system.
• Cross-reactivity with mouse and rat is reported; for non-human samples, verify recognition using appropriate tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers and researchers, providing IVD raw materials for innate immunity and infectious disease applications. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your development from concept to clinic. For NLRX1-related research and assay development, CamelBio delivers reliable reagents and technical consultation to accelerate your project.