NLRP1 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - Q9C000

Core Product Specifications and Parameters

Biological Background: NLRP1 Function and Localization

• NLRP1 (NACHT, LRR and PYD domains-containing protein 1) is a multidomain protein also known as CARD7, DEFCAP, NAC, and NALP1. It serves as the sensor component of the NLRP1 inflammasome, a supramolecular complex that assembles in the cytosol in response to pathogen-associated signals and mediates pyroptosis and inflammatory cytokine release. Related references: PMID:12191486, PMID:17349957, PMID:22665479, PMID:22801494, PMID:33852854, PMID:35594856
• Upon activation, NLRP1 recruits pro-caspase-1 (proCASP1) via the adaptor PYCARD/ASC, triggering CASP1 autoproteolysis. Active CASP1 cleaves pro-IL1B, pro-IL18, and gasdermin-D (GSDMD), leading to cytokine secretion and pyroptotic cell death. In GSDMD-deficient contexts, NLRP1 inflammasomes can engage CASP8 and gasdermin-E (GSDME). Related references: PMID:12191486, PMID:17349957, PMID:22665479, PMID:33852854, PMID:35594856
• NLRP1 detects diverse pathogen-associated and damage-associated signals: human rhinovirus 3C proteases cleave NLRP1 to activate it; long double-stranded RNA (dsRNA) acts as a direct sensor; UV-B irradiation induces ribosome collisions that activate NLRP1 via MAP3K20 phosphorylation; and the dipeptidyl peptidase inhibitor Val-boroPro also triggers the inflammasome. Related references: PMID:33093214, PMID:33243852, PMID:35857590
• NLRP1 is constitutively processed within its FIIND domain, yielding non-covalently associated N- and C-terminal fragments that maintain an autoinhibited state. Pathogen- or damage-derived signals induce ubiquitination and proteasomal degradation of the N-terminal fragment, liberating the C-terminal fragment to oligomerize and nucleate inflammasome formation. Related references: PMID:33093214, PMID:33731929, PMID:33731932
• Tissue expression is broad, with high levels in primary immune cells (neutrophils, monocytes/macrophages, dendritic cells, lymphocytes) and in glandular epithelia of the gastrointestinal, respiratory, and reproductive tracts. It is also detected in skin (keratinocytes, fibroblasts, hair follicles), brain neurons, and testicular germ cells. Related references: PMID:11113115, PMID:17164409, PMID:15285719, PMID:23349227, PMID:27662089
• Subcellularly, NLRP1 localizes to the cytoplasm, cytosol, and nucleus, and it is recruited to the inflammasome complex upon activation. It binds ATP and exhibits ATPase activity, which may be required for its function. Related references: PMID:11113115, PMID:15212762, PMID:33243852
• Key biological roles include innate immunity, inflammatory response, antiviral defense, and host–virus interaction. Mutations in NLRP1 are associated with autoinflammatory diseases and ectodermal dysplasia.

Experimental Guidance and Technical Tips

• For Western blotting, NLRP1 has a predicted molecular weight of ~166 kDa. Use a low-percentage polyacrylamide gel (e.g., 6–8%) and extended wet transfer times (overnight at low voltage) to ensure efficient transfer of this large protein. Include a high-molecular-weight prestained marker as a reference.
• For IHC-P, antigen retrieval (citrate or EDTA buffer, heat-induced) is recommended. Validate the antibody in your tissue of interest (human, mouse, or rat) with appropriate positive and negative controls, and titrate to minimize background.
• For IF/ICC, optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to allow access to the predominantly cytoplasmic/nuclear antigen. Co-staining with organelle markers can help confirm localization.
• As a polyclonal antibody, each lot may exhibit slight variations; we recommend pilot experiments to establish optimal working dilutions for your specific assay and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, antibody pairs, and ancillary reagents under one roof. This NLRP1 rabbit polyclonal antibody supports innate immunity and inflammasome research, and we can assist with custom-sourcing rare targets, bulk orders, and technical consultation to accelerate your diagnostic development. Our commitment is to bridge your concept-to-clinic journey with reliable, research-ready materials.