NFYA Rabbit mAb for WB, IP, IHC-P, ChIP, ELISA - P23511

Core Product Specifications and Parameters

Biological Background: NFYA Function and Localization

• NFYA encodes the alpha subunit of the heterotrimeric NF-Y transcription factor, also designated CAAT box DNA-binding protein subunit A (HAP2/CBF-B).
• NF-Y specifically binds the 5'-CCAAT-3' motif in target gene promoters, functioning as either a transcriptional activator or repressor depending on cofactor interactions.
• It directly upregulates the core circadian clock gene BMAL1, linking NF-YA to biological rhythm regulation.
• Localizes exclusively to the nucleus.
• Post-translational modifications include phosphorylation, and the protein is subject to alternative splicing.
• Relevant keywords: DNA-binding, activator, phosphoprotein, alternative splicing, biological rhythms, transcription regulation.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the C-terminal region (aa 262–332) of human NFYA, which is highly conserved across human, mouse, and rat.
• Based on the predicted molecular weight of ~34–37 kDa, standard SDS-PAGE and Western blotting conditions can be utilized, but validate using control lysates from relevant species/tissues.
• For chromatin immunoprecipitation (ChIP), nuclear extraction and optimized crosslinking are recommended due to the transcription factor’s nuclear localization.
• Performance in ELISA may require careful coating antigen selection (recombinant protein or synthetic peptide); titrate antibody concentration accordingly.
• Always include appropriate positive and negative controls, especially when testing less-characterized samples or species.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and researchers with high-quality antibodies against key transcription regulators like NFYA. We offer validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target materials—bridging the gap from concept to clinic.