NFKB1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor, which is cotranslationally processed by the proteasome to generate the active p50 subunit (PubMed:1423592). Related references: PMID:1423592
• The p50 subunit forms functional NF-kB dimers by associating with other Rel family members, most notably the p65-p50 heterodimer, which acts as a potent transcriptional activator (PubMed:1740106, PubMed:7830764). Related references: PMID:1740106, PMID:7830764
• NF-kB dimers specifically bind to the kappa-B consensus DNA sequence (5'-GGRNNYYCC-3') located in enhancer regions of genes governing immune responses, acute-phase reactions, inflammation, and cell survival.
• In unstimulated cells, NF-kB is sequestered in the cytoplasm by inhibitor I-kB proteins; upon activation by diverse stimuli, I-kB is phosphorylated and degraded, freeing NF-kB to enter the nucleus and regulate target gene expression.
• The full-length p105 also functions as an I-kB-like inhibitor, retaining associated NF-kB proteins in the cytoplasm and modulating MAPK signaling by repressing MAP3K8 until its degradation releases active MAP3K8.
• Key post-translational modifications of NFKB1 include phosphorylation, acetylation, ubiquitination, and S-nitrosylation, which fine-tune its transcriptional activity and stability.
• Subcellular localization is primarily cytoplasmic in resting cells and nuclear upon pathway activation.

Experimental Guidance and Technical Tips

• This antibody, raised against a peptide within the N-terminal region common to both p105 and p50, is expected to detect both forms; in WB, expect bands at ~105 kDa (p105) and ~50 kDa (p50) depending on cell type and stimulation.
• For IHC-P and IF/ICC, antigen retrieval conditions and fixation methods may require optimization; consider testing both cytoplasmic and nuclear staining patterns.
• Verify antibody specificity using appropriate controls, such as siRNA-mediated knockdown or knockout cell lines.
• For ELISA applications, confirm that the antibody recognizes native protein conformations in your sample matrix.
• Adjust blocking and antibody dilution conditions empirically for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. For NF-kB pathway research and diagnostic development, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your projects.