NFATC2 Rabbit pAb - Q13469

Core Product Specifications and Parameters

Biological Background: NFATC2 Function and Localization

• NFATC2 (also known as NFAT1, NF-ATp, JCOSL, NFATP) is a member of the NFAT transcription factor family, characterized by a DNA-binding domain and calcineurin-regulated nuclear localization.
• It plays a critical role in the inducible expression of cytokine genes in T-cells, including IL-2, IL-3, IL-4, TNF, and GM-CSF. Related references: PMID:15790681
• NFATC2 promotes invasive cell migration by activating GPC6 expression and the WNT5A signaling pathway. Related references: PMID:21871017
• It is involved in the negative regulation of chondrogenesis. Related references: PMID:35789258
• In resting cells, NFATC2 is predominantly cytoplasmic; upon T-cell receptor stimulation, it is dephosphorylated by calcineurin and translocates to the nucleus to activate transcription.
• The protein undergoes phosphorylation and ubiquitination, influencing its activity and stability.
• NFATC2 is broadly expressed in tissues including thymus, spleen, heart, testis, brain, placenta, muscle, and pancreas, with specific isoforms showing differential expression patterns.
• It is recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains, coupling store-operated Ca(2+) influx to calcineurin and NFAT activation.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a recombinant fusion protein covering amino acids 1–300 of human NFATC2. This region includes the N-terminal part of the protein, which may contribute to epitope exposure. When planning detection experiments, consider that the antibody may recognize isoforms containing this sequence.
• Based on the supplier's reported applications (WB, IF/ICC, IF-P, IHC-P, ELISA), the antibody is suitable for multiple detection platforms. For Western blot, a band around 100 kDa is expected. For immunostaining, fixation and permeabilization conditions may need optimization to detect nuclear translocated NFATC2.
• Cross-reactivity is reported for human, mouse, and rat, but validation in the specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to support diagnostic development. This NFATC2 Rabbit pAb aids T-cell signaling research, and CamelBio can assist with sourcing complementary reagents or bulk orders to streamline your workflow from early research to clinical application.