NFASC Rabbit pAb - O94856

Core Product Specifications and Parameters

Biological Background: NFASC (Neurofascin) Function and Localization

• Neurofascin (NFASC) is a cell adhesion protein and ankyrin‑binding partner belonging to the immunoglobulin superfamily, expressed as multiple isoforms due to alternative splicing.
• It plays critical roles in neurite extension, axonal guidance, synaptogenesis, and myelination, mediating neuron–glial cell interactions during nervous system development.
• NFASC is localized to the cell membrane and concentrates at paranodal septate junctions, where it contributes to the organization of axo‑glial contact sites.
• The protein contains immunoglobulin‑like domains and fibronectin type III repeats, characteristic of cell adhesion molecules, and is modified by glycosylation, phosphorylation, and intermolecular disulfide bonds.
• Mutations in NFASC are associated with neurodevelopmental disorders (NEDCPMD), underscoring its importance in human brain function.
• The NFASC gene encodes a protein of approximately 150 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 830–1100, targeting a region that may be present in multiple NFASC isoforms; confirm detection of intended variants using isoform‑specific controls or databases.
• For Western blotting, sample preparation under reducing conditions is recommended to resolve the approximately 150 kDa band and to reduce higher‑order oligomers caused by disulfide bonds.
• Although validated for human, mouse, and rat, it is advisable to verify reactivity and specificity in your particular tissue or cell line, as expression levels and isoform patterns can vary.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal‑to‑noise ratio; consider using the recombinant immunogen as a positive control.
• Because neurofascin is a transmembrane protein, inclusion of membrane protein extraction protocols may improve detection sensitivity in tissue homogenates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of validated IVD raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti‑NFASC polyclonal antibody exemplifies our capacity to provide high‑quality tools for neuroscience and cell adhesion research. Our sourcing services extend to bulk‑scale supply, custom antibody development, and supply chain consolidation—bridging the gap from target discovery to clinical assay development.