NF-κB2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00653

Core Product Specifications and Parameters

Biological Background: NF-κB2 Function and Localization

• NF-κB2 encodes the p100 precursor protein, which is proteolytically processed to generate the active p52 DNA-binding subunit.
• NF-κB2 is a critical component of the NF-kappa-B transcription factor complex, orchestrating gene expression in inflammation, immune responses, cell survival, and oncogenesis.
• The non-canonical NF-κB pathway involves IKKα-mediated phosphorylation of p100, leading to its processing and formation of RelB-p52 heterodimers that activate transcription.
• NF-κB2 possesses dual functions: the p100 form retains NF-κB complexes in the cytoplasm via ankyrin repeats, while p52 translocates to the nucleus to regulate target genes.
• Subcellular localization is primarily cytoplasmic, with stimulus-dependent nuclear translocation of the active p52-containing complexes.
• Alternative splicing yields isoform p49, which synergizes with p65 to enhance HIV enhancer activity.
• Chromosomal rearrangements involving NFKB2 (e.g., LYT10) are associated with lymphoid malignancies, highlighting its proto-oncogenic potential.
• Keywords: NF-κB2 is classified as a proto-oncogene, transcriptional activator/repressor, and is subject to ubiquitination and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 690-899) lies within the C-terminal region of p100; thus, the antibody is predicted to recognize the full-length p100 precursor (~97 kDa) but not the cleaved p52 subunit.
• For Western blotting, expect a band at approximately 97 kDa corresponding to p100; stimulation of the non-canonical pathway may increase band intensity.
• In immunohistochemistry and immunofluorescence, optimize antigen retrieval and include isotype controls to verify signal specificity.
• Cross-reactivity with mouse and rat has been reported; however, titration and validation in the relevant tissue or cell line are recommended.
• For ELISA, perform checkerboard titrations to determine optimal antibody and antigen concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for global diagnostic manufacturers and researchers. This NF-κB2 antibody supports studies in immune signaling, inflammation, and oncology, fitting seamlessly into our portfolio of transcription factor reagents. For custom antibody development, rare target sourcing, or technical consultation, contact CamelBio to accelerate your project from concept to clinic.