NF-κB2 Rabbit pAb – Polyclonal Antibody for WB, IF/ICC, ELISA - Q00653

Core Product Specifications and Parameters

Biological Background: NF-κB2 Function and Localization

• NF-κB2 encodes the p100 precursor and its processed p52 subunit, a component of the NF-κB transcription factor family.
• It forms homo- or heterodimers with Rel proteins (RELA, RELB, REL) to regulate genes involved in immune response, inflammation, cell growth, and apoptosis.
• In the non-canonical NF-κB pathway, p100 undergoes proteasomal processing to p52, leading to formation of the RelB-p52 activator complex.
• p52-p52 homodimers act as transcriptional repressors, while p52-RELA and p52-RELB complexes can activate target genes.
• The protein localizes to both the cytoplasm and nucleus; cytoplasmic p100 retains associated NF-κB proteins, while p52 translocates to the nucleus upon activation.
• Post-translational modifications include phosphorylation, ubiquitination, and processing events that regulate its activity (UniProt keywords: Phosphoprotein, Isopeptide bond, Ubl conjugation).
• NF-κB2 has been implicated in circadian clock regulation by repressing CLOCK-BMAL1 activity together with RELB.
• Genetic rearrangements and mutations in NFKB2 are associated with immunodeficiencies such as CVID10.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 690-899 of human NF-κB2 (p100 C-terminal region), which may recognize both the p100 precursor and the p52 processed form.
• For Western blot (WB), a band around 97 kDa (p100) and a smaller band around 52 kDa (p52) may be observed, depending on cellular processing.
• Consider using appropriate cell lysates or stimuli that activate the non-canonical NF-κB pathway to assess p100 processing to p52.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization should be optimized to detect both cytoplasmic and nuclear pools.
• Cross-reactivity with mouse samples has been reported; validate in the relevant sample system.
• ELISA may be suitable for detecting recombinant or endogenous NF-κB2 protein; titration of antibody concentration is recommended.

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CamelBio offers a comprehensive portfolio of IVD raw materials, including this NF-κB2 polyclonal antibody, to support diagnostic assay development and research. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for inflammation and immune response targets. Our team facilitates rare target raw-material procurement, helping you bridge from concept to clinical application efficiently.