NF-kB p65/RelA Rabbit Polyclonal Antibody - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• NF-kB p65 (RELA) is a critical subunit of the NF-kB transcription factor complex, which regulates immune and inflammatory responses, cell growth, differentiation, and apoptosis.
• The most abundant NF-kB complex is the RELA-NFKB1 heterodimer, but RELA can also form homodimers or heterodimers with REL, each binding kappa-B sites with distinct affinity and specificity.
• RELA is essential for cytokine gene expression in T-cells. Related references: PMID:15790681
• Subcellular localization is tightly controlled: RELA is retained in the cytoplasm by I-kB inhibitors and translocates to the nucleus upon activation.
• RELA is a key transcription factor mediating the interferon response during SARS-CoV-2 infection. Related references: PMID:33440148
• Post-translational modifications such as phosphorylation, acetylation, methylation, ubiquitination, and S-nitrosylation modulate RELA activity and stability.
• Alternative splicing and chromosomal rearrangements of RELA have been associated with disease variants.
• The protein is involved in host-virus interactions and is a proteomics-identified reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 450-550) of human p65/RelA, which may detect full-length protein and some isoforms. Validate specificity with relevant positive and negative controls.
• For Western blotting, a predominant band around 60 kDa is expected. The use of nuclear extracts can enhance detection following NF-kB pathway activation.
• In immunofluorescence and immunohistochemistry, nuclear localization is typically observed upon stimulation. Include appropriate unstimulated controls and confirm with counterstains.
• Cross-reactivity with mouse and rat enables utility in rodent models; consider species-specific validation for your experimental system.
• For ELISA applications, the antibody may be used as a capture or detection reagent; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, covering everything from optimized monoclonal and polyclonal antibodies to bulk ancillary reagents. This anti-NF-kB p65/RelA antibody supports research in inflammation, immunity, and related disease models, and CamelBio can assist with validated antibody pairs, rare target sourcing, and technical consultation to accelerate your diagnostic development from concept to clinic.