NF-kB p65/RelA Mouse Monoclonal Antibody for WB, ELISA - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• NF-kB p65 (RELA) is the p65 subunit of the pleiotropic NF-kappa-B transcription factor complex. It belongs to the NF-kappa-B/Rel protein family, which also includes RELB, NFKB1/p105/p50, REL, and NFKB2/p52.
• RELA forms homo- or heterodimers with other family members; the RELA-NFKB1 heterodimer is the most abundant. These dimers bind specific DNA kappa-B sites to activate transcription of target genes involved in inflammation, immunity, cell growth, and apoptosis.
• The protein shuttles between the cytoplasm and nucleus. In unstimulated cells, it is retained in the cytoplasm via interaction with I-kappa-B inhibitor proteins. Upon stimulation, I-kappa-B is phosphorylated and degraded, freeing RELA to translocate to the nucleus and regulate gene expression.
• RELA is essential for cytokine gene expression in T-cells.
  Related references: PMID:15790681
• RELA acts as a key transcription factor controlling the interferon response during SARS-CoV-2 infection.
  Related references: PMID:33440148
• The protein undergoes multiple post-translational modifications including acetylation, phosphorylation, methylation, S-nitrosylation, and ubiquitination, which modulate its activity, stability, and interactions. Associated keywords also include chromosomal rearrangement, disease variant, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen is recombinant full-length human NF-kB p65, suggesting the antibody recognizes conserved epitopes across species. Cross-reactivity with mouse and rat has been confirmed, making it suitable for studies in these common model organisms.
• For Western blot, the expected molecular weight is approximately 60 kDa. Consider using appropriate positive and negative controls to validate specificity in your sample type and treatment conditions.
• For ELISA applications, optimize coating and detection conditions empirically. Pairing with another NF-kB p65 antibody may enable sandwich ELISA development; validate the pair in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers, laboratories, and research institutes by offering integrated access to validated IVD raw materials, technical services, and consulting. This NF-kB p65/RelA monoclonal antibody serves as a critical tool for studying inflammation, oncology, and immunology pathways. Beyond antibodies, CamelBio supports your development pipeline with antibody pairs, bulk ancillary reagents, and custom rare-target sourcing—helping you move confidently from concept to clinic.