Neurofilament M Rabbit mAb for WB, IF/ICC, ELISA - P07197

Core Product Specifications and Parameters

Biological Background: Neurofilament M Function and Localization

• Neurofilament M (NFM, NEF3) is a 102 kDa intermediate filament protein encoded by the NEFM gene in humans. It is also known as neurofilament medium polypeptide and neurofilament triplet M protein.
• As a core component of neurofilaments, NFM cooperates with neurofilament light (NEFL) and heavy (NEFH) polypeptides to maintain neuronal axon caliber and structural integrity.
• NFM may additionally interact with peripherin (PRPH) and alpha-internexin (INA) to form specialized neuronal intermediate filament networks.
• The protein localizes predominantly to the cytoplasm and cytoskeleton, with enrichment in neuronal cell projections such as axons and the perikaryon.
• NFM is subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, and glycosylation, which regulate filament assembly, stability, and interactions.
• Alternative splicing of NEFM generates multiple isoforms, potentially contributing to functional diversity in different neuronal populations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 600–700 of human NFM; this C-terminal region may contain unique epitopes, but verify specificity using knockout or knockdown controls.
• For Western blot, a band at ~102 kDa is expected; use appropriate loading controls and validate with known neurofilament-expressing samples.
• In immunofluorescence/ICC, anticipate filamentous staining patterns in neuronal cells; optimize fixation and permeabilization conditions for your system.
• When using this antibody in ELISA, consider its monoclonal nature for consistent batch-to-batch performance; validate linearity and sensitivity in your assay format.
• The reported cross-reactivity is limited to human; if testing other species, conduct sequence alignment of the immunogen region and consider experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies for neuronal markers. Beyond this anti-Neurofilament M antibody, we offer matched antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to support your neurofilament assay development. Our global supply network also facilitates sourcing of rare-target raw materials, helping you accelerate from concept to clinical application.