Neurofilament H Rabbit Monoclonal Antibody for WB - P12036

Core Product Specifications and Parameters

Biological Background: Neurofilament H Function and Localization

• Neurofilament H (NFH), also known as 200 kDa neurofilament protein or neurofilament triplet H protein, is encoded by the NEFH gene and belongs to the intermediate filament family.
• NEFH is a key component of the neuronal cytoskeleton and, together with NEFL and NEFM, maintains neuronal caliber; it has a unique and essential role in mature axons that cannot be fully compensated by the two smaller neurofilament proteins.
• It may also cooperate with the neuronal intermediate filament proteins peripherin (PRPH) and internexin (INA) to form robust filamentous networks in neurons.
• Subcellular localization is predominantly cytoplasmic, with enrichment in the cytoskeleton, cell projections, and particularly axons.
• The protein is subject to extensive post-translational modifications, including phosphorylation, and is implicated in several neurodegenerative diseases such as Charcot-Marie-Tooth disease type 2CC and amyotrophic lateral sclerosis.
• UniProt lists the molecular weight as approximately 112 kDa (111838 Da), which is consistent with the commonly reported size of the full-length protein.

Experimental Guidance and Technical Tips

• Given the immunogen spans the C-terminal tail region (aa 873–1020), this antibody is expected to detect full-length NEFH and may not cross-react with truncated isoforms lacking this region. Consider validating in your sample system.
• For Western blotting, a band around 112 kDa is anticipated. Use appropriate protein loading controls and sample preparation to prevent degradation of this large protein.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization protocols to preserve the filamentous structure; strong axonal staining is expected in neuronal tissues.
• As a rabbit monoclonal antibody, it provides high batch-to-batch consistency; use appropriate anti-rabbit secondary reagents for detection.
• Cross-reactivity with mouse and rat NEFH makes it suitable for translational neuroscience studies using rodent models; confirm reactivity if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, connecting diagnostic manufacturers and research labs with high-quality Neurofilament H antibodies and complementary reagents for neuronal biomarker assays. From validated monoclonal and polyclonal antibodies to bulk ancillary reagents and rare target raw-material sourcing, we support your workflow from concept to clinic. Leverage our expertise to accelerate your neuro-diagnostic development.