NeuroD1 Rabbit Monoclonal Antibody for WB and ELISA - Q13562

Core Product Specifications and Parameters

Biological Background: Neurogenic differentiation factor 1 (NeuroD1) Function and Localization

• NeuroD1, also known as BETA2, bHLHa3, or MODY6, is a class A basic helix-loop-helix (bHLH) transcription factor that acts as a transcriptional activator by binding to E-box consensus sequences (5'-CANNTG-3') in target gene promoters.
• It associates with the p300/CBP coactivator complex to stimulate the expression of genes such as secretin and the cyclin-dependent kinase inhibitor CDKN1A, thereby influencing cell differentiation and cell cycle regulation.
• NeuroD1 plays a critical role in the differentiation of multiple cell types, including early retinal ganglion cells, inner ear sensory neurons, cerebellar and hippocampal granule cells, pancreatic endocrine islet cells, and enteroendocrine cells of the small intestine.
• Together with PAX6 or SIX3, NeuroD1 is required for amacrine cell fate specification in the retina, and it is also essential for dendrite morphogenesis and maintenance in the cerebellar cortex.
• The protein associates with chromatin at enhancer regulatory elements of key neurogenesis transcriptional regulators, modulating gene expression programs during development.
• NeuroD1 shuttles between the cytoplasm and nucleus, with its activity regulated by phosphorylation; it is implicated in diseases such as diabetes mellitus (e.g., MODY6) due to its role in pancreatic beta-cell development.
• As a phosphoprotein and DNA-binding transcription factor, NeuroD1 is classified under multiple UniProt keywords including activator, developmental protein, neurogenesis, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1-100) of human NeuroD1, which may contribute to the antibody's specificity for the target.
• In Western blot (WB) applications, a band at approximately 40 kDa is expected; consider using whole-cell lysates from neuronal or pancreatic tissues for optimal detection.
• For ELISA, the peptide-based immunogen suggests potential suitability for direct or sandwich ELISA formats; optimization of coating conditions and antibody concentration is recommended.
• Cross-reactivity with mouse NeuroD1 is reported; this is likely due to high sequence conservation across species, but experimental validation in mouse samples is advised.
• As a rabbit monoclonal antibody, it typically offers high batch-to-batch consistency; however, each new lot should be titrated and validated in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting manufacturers and research institutes from concept to clinic. For research into neurogenesis, diabetes, and transcription factor biology, we offer a curated portfolio of validated antibodies, including monoclonal and polyclonal options, along with ancillary reagents and rare-target sourcing. Our technical services and consulting further assist in streamlining your assay development and scale-up processes.