NEURL1B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - A8MQ27

Core Product Specifications and Parameters

Biological Background: NEURL1B Function and Localization

• NEURL1B encodes an E3 ubiquitin-protein ligase, a member of the neuralized family of RING-type E3 ligases, and is also known as Neuralized-2 (NEUR2), NEURL3, or RNF67B.
• The protein functions in the Notch signaling pathway by modulating the stability and activity of several Notch ligands, thereby influencing downstream signaling events.
• NEURL1B is localized to the cytoplasm, consistent with its role in substrate ubiquitination and protein turnover.
• Tissue expression is highest in the brain, prostate, and small intestine, with higher levels in fetal brain compared to adult; in adult brain, strong expression is observed in the olfactory system, cerebellar cortex, optic nerve, and frontal lobe.
• The protein contains a RING-type zinc finger and NEUZ domains, indicative of its E3 ubiquitin ligase activity and metal-binding properties.
• Multiple isoforms exist due to alternative splicing, and the protein is subject to phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 270–440 of human NEURL1B (NP_001136123.1). This region may recognize multiple isoforms if the epitope is conserved; validate isoform detection in your system.
• Recommended applications include Western blot (WB), immunohistochemistry-paraffin (IHC-P), and ELISA. Optimize antibody dilutions for each assay using appropriate positive and negative controls.
• For WB, the predicted molecular weight of NEURL1B is approximately 59 kDa; confirm band specificity against lysates from tissues with high expression (e.g., brain).
• As a polyclonal antibody, it may exhibit batch-to-batch variation; consider lot-specific validation for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes this NEURL1B polyclonal antibody, suitable for Notch pathway research and E3 ligase studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your assay development from concept to clinic.