NDUFS4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43181

Core Product Specifications and Parameters

Biological Background: NDUFS4 Function and Localization

• NDUFS4 encodes the 18 kDa accessory subunit of mitochondrial NADH dehydrogenase (Complex I), also known as CI-18 kDa, CI-AQDQ, or NDUFS4.
• It is a non-catalytic subunit, believed to play a structural or assembly role in Complex I, which transfers electrons from NADH to ubiquinone in the respiratory chain.
• The protein localizes to the mitochondrial inner membrane, on the matrix side.
• Post-translational modifications include phosphorylation (Keyword: Phosphoprotein), which may regulate its function or complex assembly.
• Pathogenic variants in NDUFS4 cause mitochondrial complex I deficiency, nuclear type 1 (MC1DN1), a primary mitochondrial disease characterized by severe neurological symptoms.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length mature human NDUFS4 (amino acids 1-175), so the antibody is expected to recognize the native protein in correctly folded Complex I.
• For western blotting, a specific band around 20 kDa is anticipated in human, mouse, and rat samples; validate band identity using knockdown or knockout controls.
• In immunohistochemistry (IHC-P), consider optimizing antigen retrieval and primary antibody dilution for mitochondrial detection in tissue sections.
• For ELISA applications, pair with a suitable detection antibody and confirm linearity and specificity using recombinant NDUFS4 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For mitochondrial complex I research, CamelBio ensures reliable supply of anti-NDUFS4 antibodies and can assist in sourcing rare-target raw materials to accelerate your assay development and diagnostic kit production.