NDUFB5 Rabbit Polyclonal Antibody for WB, ELISA - O43674

Core Product Specifications and Parameters

Biological Background: NDUFB5 Function and Localization

• NDUFB5 encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It is believed not to be directly involved in catalysis.
• Complex I functions in the transfer of electrons from NADH to the respiratory chain, with the immediate electron acceptor believed to be ubiquinone.
• The NDUFB5 protein localizes to the mitochondrial inner membrane.
• The protein is classified under electron transport and respiratory chain pathways, with a transmembrane domain as indicated by UniProt keywords.
• Alternative splicing of the NDUFB5 gene may generate multiple transcript variants.
• The protein's 3D structure has been determined, providing insights into its structural role within Complex I.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-189 of human NDUFB5, a region likely exposed in the mature protein. This may confer specificity for detecting the protein in denaturing conditions such as Western blot.
• Given the polyclonal nature, the antibody can be expected to recognize multiple epitopes, which may enhance signal sensitivity.
• For Western blot, the predicted molecular weight is approximately 22 kDa; validate detection in the relevant sample system (e.g., human, mouse, or rat cell lysates).
• For ELISA, the recombinant fusion protein used as immunogen can be considered as a positive control or standard in assay development.
• Since NDUFB5 is a mitochondrial inner membrane protein, consider enriching mitochondrial fractions to improve detection in complex samples.
• Cross-reactivity with mouse and rat is predicted based on sequence homology; optimal conditions should be determined empirically.

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CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including antibodies for mitochondrial and respiratory chain targets like NDUFB5. We support your projects from concept to clinic with validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For rare or specialized targets, our global sourcing network ensures reliable supply.