NDUFB2 Rabbit pAb for WB, ELISA - O95178

Core Product Specifications and Parameters

Biological Background: NDUFB2 Function and Localization

• NDUFB2 encodes the NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, also known as Complex I-AGGG (CI-AGGG) or NADH-ubiquinone oxidoreductase AGGG subunit.
• It is an accessory subunit of mitochondrial Complex I that is believed to be non-catalytic, playing a structural or assembly role rather than direct participation in electron transfer.
• Complex I is the first and largest enzyme of the mitochondrial respiratory chain, catalyzing the transfer of electrons from NADH to ubiquinone, coupled with proton translocation across the inner membrane.
• NDUFB2 localizes exclusively to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• The protein contains a mitochondrial transit peptide that directs its import and proper folding within the organelle.
• Structural studies have contributed to understanding the architecture of the membrane arm of Complex I, though the exact mechanistic role of NDUFB2 remains under investigation.
• No known post-translational modifications or tissue-specific variants have been reported for this subunit, indicating a relatively conserved function across tissues.

Experimental Guidance and Technical Tips

• The immunogen covers a region spanning amino acids 34-105, which likely corresponds to a mature part of the protein after cleavage of the transit peptide. Consider using this antibody in assays where the mature mitochondrial form is relevant.
• For Western blotting, a band around 12 kDa is expected for the processed protein. However, the precursor form including the transit peptide may appear slightly larger; validate in your specific sample system.
• The antibody cross-reacts with mouse and rat NDUFB2, making it suitable for comparative studies across these species. However, always confirm cross-reactivity in your experimental model.
• For ELISA, pair with a suitable detection antibody; the recombinant protein immunogen can serve as a positive control for assay optimization.
• Since NDUFB2 is a mitochondrial protein, include appropriate subcellular fractionation or organelle isolation steps to enrich the target for detection.

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